Phytochemical profiling and anti-Staphylococcus epidermidis activity of mango leaf extract using gas chromatography-mass spectrometry
DOI:
https://doi.org/10.33503/ebio.v10i01.1177Keywords:
Antibacterial, mango, MIC, Phenols, Staphylococcus epidermidisAbstract
Staphylococcus epidermidis is increasingly recognized for its resistance to certain antibiotics, posing a significant threat due to its potential to cause infections. Plant extracts have emerged as promising alternatives owing to their diverse phytochemical composition. This study aimed to evaluate the antibacterial efficacy of the ethanolic extract of Arumanis mango leaves (Mangifera indica L. var. arumanis) against S. epidermidis and to characterize its metabolite profile using Gas Chromatography-Mass Spectrometry (GC-MS). Employing the disc diffusion method, the extract demonstrated inhibitory activity against S. epidermidis at a concentration of 2% (2 mg/ml). Subsequent GC-MS analysis identified phenols, flavonoids, and alcohols as the predominant bioactive compounds, consistent with their known antibacterial properties. These findings suggest that Arumanis mango leaf extract holds potential as a natural antibacterial agent against S. epidermidis and warrants further investigation for incorporation into natural antibacterial formulations.
References
Admojo, F. T. (2020). Klasifikasi Aroma Alkohol Menggunakan Metode KNN. Indonesian Journal of Data
and Science, 1(2), 34-38. https://doi.org/10.33096/ijodas.v1i2.12
Asngad, A., & Nopitasari, N. (2018). Kualitas gel pembersih tangan (handsanitizer) dari ekstrak batang
pisang dengan penambahan alkohol, triklosan dan gliserin yang berbeda dosisnya. Bioeksperimen: Jurnal Penelitian Biologi, 4(2), 61-70. https://doi.org/10.23917/bioeksperimen.v4i2.6888
Chirumamilla, P., Dharavath, S. B., & Taduri, S. (2022). GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts. Bulletin of the National Research Centre, 46(1-10), 127. https://doi.org/10.1186/s42269-022-00818-9
Diso, S U., Ali, M., Mukhtar, S I., & Garba, M. (2017). antibacterial activity and phytochemical screening of Mangifera indica (mango) stem and leaf extracts on clinical isolates of methicillin resistant Staphylococcus aureus. Journal of Advances in Medical and Pharmaceutical Sciences, 13 (1): 1-6. https://journaljamps.com/index.php/JAMPS/article/view/202
Frieri, M., Kumar, K., & Boutin, A. (2017). Antibiotic resistance. Journal of infection and public health, 10(4), 369-378. https://doi.org/10.1016/j.jiph.2016.08.007
Hafsari, A. R., Cahyanto, T., Sujarwo, T., & Lestari, R. I. (2015). Uji aktivitas antibakteri ekstrak daun beluntas (Pluchea indica (l.) less.) terhadap propionibacterium acnes penyebab jerawat. Jurnal Istek, 9(1). https://journal.uinsgd.ac.id/index.php/istek/article/view/174
Ines Thabti, Walid Elfalleh, Nizar Tlili, Manel Ziadi, Maria Graça Campos & Ali Ferchichi (2014) Phenols, flavonoids, and antioxidant and antibacterial activity of leaves and stem bark of morus species, International Journal of Food Properties, 17:4, 842-854, https://doi.org/10.1080/10942912.2012.660722
Kumalasari, S., Fitriyati, L., Rahayu, T. P., & Kiromah, N. Z. W. (2024). antibacterial activity test of the combination of methanol extract of arumanis mango leaves (Mangifera indica L) and taro leaves (Colocasia esculanta L) on Escherichia coli bacteria causes of diarrhea diseases. Jurnal Pijar Mipa,19(1), 131–135. https://doi.org/10.29303/jpm.v19i1.5850
Lee E, Anjum F. Staphylococcus epidermidis Infection. (2018). [Updated 2023 Apr 27]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK563240/
Lee, J. Y., Monk, I. R., Gonçalves da Silva, A., Seemann, T., Chua, K. Y., Kearns, A. & Howden, B. P. (2018). Global spread of three multidrug-resistant lineages of Staphylococcus epidermidis. Nature Microbiology, 3(10), 1175-1185. https://doi.org/10.1038/s41564-018-0230-7
Mehmood, H., Mehmood, J., & Zulfiqar, N. (2024). Exploring the phytochemistry and pharmacology of Mangifera indica L. (Mango) leaves: A review. International Journal of Plant Based Pharmaceuticals, 4(1), 9-18, https://doi.org/10.29228/ijpbp.38 .
Meilina, N. E., & Hasanah, A. N. (2018). Review Artikel: Aktivitas antibakteri ekstrak kulit buah manggis (Garnicia mangostana L.) terhadap bakteri penyebab jerawat. Farmaka, 16(2) 322-328. https://jurnal.unpad.ac.id/farmaka/article/view/17550/pdf
Musetti, A., Paderni, K., Fabbri, P., Pulvirenti, A., Al‐Moghazy, M., & Fava, P. (2014). Poly (vinyl alcohol)‐based film potentially suitable for antimicrobial packaging applications. Journal of food science, 79(4), E577-E582. https://doi.org/10.1111/1750-3841.12375
Ningsih, N.; Zusfahair, Z.; Hermawan, D.; Anggraeni, W.; Aboul-Enein, H. (2023). Formulation and characterization oinment of arum manis mango (Mangifera indica L.) leaves methanol extracts as an antibacterial against propionibacterium acnes. Curr. Bioact. Compd. 2020, 16, 67–75. http://dx.doi.org/10.2174/1573407216666200214100855
Ningsih, D R; Riapanita, A; Zusfahair; Sulaeman, U; Khoeriyah, I. (2021). Ointment formulation of arumanis mango (Mangifera indica L.) Leaf extract with chitosan tripoliphosphate matrix as antibacterial. Molekul,18 (1), 11-20.doi: https://doi.org/10.20884/1.jm.2023.18.1.5725.
Nugraha, A. C., Prasetya, A. T., & Mursiti, S. (2017). Isolasi, identifikasi, uji aktivitas senyawa flavonoid sebagai antibakteri dari daun mangga. Indonesian Journal of Chemical Science. www.journal.unnes.ac.id/sju/ijcs/article/download/12087/8628/
Othman L, Sleiman A and Abdel-Massih RM (2019). Antimicrobial activity of polyphenols and alkaloids in middle eastern plants.front. Microbiol. 10 pp 1-28 :911. https://doi:10.3389/fmicb.2019.00911
Olusola, S. E., Olorunsola, R. A., & Omileye, O. E. (2019). Therapeutic effects of
mango (Mangifera indica) and cashew (Anacardium occidentale) leaves
extracts against certain pathogenic bacterial strains from Clarias
gariepinus. Ife Journal of Science, 21(2), 357-366. https://doi.org/10.4314/ijs.v21i2.9
Putri, Sastia P., Yasumune Nakayama, Fumio Matsuda, Takato Uchikata, Shizu Kobayashi, Atsuki Matsubara, and Eiichiro Fukusaki. (2013). Current metabolomics: practical applications. Journal of Bioscience and Bioengineering, 115(6):579–89. https://doi:10.1016/j.jbiosc.2012.12.007
Shah, Ashfaq & Gupta, Amit & Rehman, Aqueel & Kapoor, Sanchita & Kaur, Harmanpreet & Rohilla, Bharat & Rashmi, Kumari & Bajpai, AB. (2023). GC-MS analysis of phytoactive compounds, antioxidant and antibacterial activity of citrullus lanatus seeds. Biomedical and Pharmacology Journal. 16. 73-86. https://doi.10.13005/bpj/2589
Shah, K., M. Patel, R. Patel, and P. Parmar. (2010). Mangifera indica (Mango). Pharmacognosy Reviews 4(7):42–48. https://doi.org/10.4103/0973-7847.65325
Siregar, A. F., Sabdono, A., & Pringgenies, D. (2012). Potensi antibakteri ekstrak rumput laut terhadap bakteri penyakit kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus. Journal of Marine Research, 1(2), 152-160. https://doi.org/10.14710/jmr.v1i2.2032
Surjowardojo, P., T. E. Susilorini, G. R. B. Sirait. (2015). Daya Hambat Dekok
Kulit Apel Arumanis (Malus sylvestrs Mill.) Terhadap Pertumbuhan
Staphylococcus aureus dan Pseudomonas Sp. Penyebab Mastitis pada
Sapi Perah. Jurnal Ternak Tropika. 16(2): 40-48. https://doi.org/10.21776/ub.jtapro.2015.016.01.11
Swamy, M. K., Arumugam, G., Kaur, R., Ghasemzadeh, A., Yusoff, M. M., & Sinniah, U. R. (2017). GC‐MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evidence‐Based Complementary and Alternative Medicine, 2017(1), pp 1-10 1517683. https://doi.org/10.1155/2017/1517683
Tollenaere, M.D.; Boira, C.; Chapuis, E.; Lapierre, L.; Jarrin, C.; Robe, P.; Zanchetta, C.; Vilanova, D.; Sennelier-Portet, B.; Martinez, J. (2022). Action of mangifera indica leaf extract on acne-prone skin through sebum harmonization and targeting c. Acnes. Molecules 2022(27): 4769. Pp 1-14 https://doi.org/10.3390/molecules27154769
Utami, L. A., & Putri, D. H. (2020). The effect of ethanol solvent concentration on antimicrobial activities the extract of andalas endophytic bacteria (Morus Macroura Miq.) Fermentation Product. Eksakta: Berkala Ilmiah Bidang MIPA, 21(1), 1-6. http://dx.doi.org/10.24036/eksakta/vol21-iss1/210
Vuong, Cuong & Otto, Michael. (2002). Staphylococcus epidermidis infections. Microbes and infection / Institut Pasteur. 4. 481-489. https://doi:10.1016/S1286-4579(02)01563-0
Yodha, A W; Badia, E; Musdalipah; Setiawan, M; Daud, N S; Fusvita, A; Fristiohady, A; Sahidin. (2023) Essential oils of alpinia monopleura and their antibacterial and antioxidant activity. Molekul, 18 (1), 80-88, doi: https://doi.org/10.20884/1.jm.2023.18.1.6265.
Zakiah, R. M., Elsyana, V., & Marcellia, S. (2023). Perbandingan Aktivitas Anti Bakteri Ekstrak Etanol Dan Ekstrak N-Heksana Daun Mangga Arum Manis (Mangifera indica L. var. arum manis) Terhadap Propionabacterium Acnes. Jurnal Ilmiah Wahana Pendidikan, 9(1), 367-376. http://dx.doi.org/10.24036/eksakta/vol21-iss1/210
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